mmp 13 (Proteintech)
Structured Review

Mmp 13, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 803 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mmp+13/MMP13+Antibody/pmc13031041-114-23-26
Average 96 stars, based on 803 article reviews
Images
1) Product Images from "Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression"
Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression
Journal: Non-coding RNA Research
doi: 10.1016/j.ncrna.2025.11.007
Figure Legend Snippet: Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.
Techniques Used: In Vivo, Staining, TUNEL Assay, Immunohistochemistry, Expressing
Related Articles
Incubation:Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression Article Snippet: For immunohistochemistry (IHC), tissue sections underwent sequential antigen retrieval and blocking using a high-power microwave treatment, endogenous peroxidase blocker (Biocare Medical, USA), and 5 % sheep serum (Beyotime, China). .. Sections were then incubated with primary antibodies against Collagen II (28459-1-AP; 1:200; Proteintech, USA), Aggrecan (13880-1-AP; 1:100; Proteintech), MMP-3 (17873-1-AP; 1:200; Proteintech), and Article Title: Hypoxia-preconditioned bone marrow mesenchymal stem cell-derived exosomes ameliorate knee osteoarthritis by promoting cartilage regeneration and alleviating pain in rats Article Snippet: .. The sections were blocked with 10 % fetal bovine serum (FBS) for 1 h at room temperature, then incubated overnight at 4 °C with primary antibodies against iNOS (Servicebio, China), COX2 (ProteinTech Group, China), Collagen II (Bioss, China), aggrecan (Bioss, China), Article Title: Hypoxia-preconditioned bone marrow mesenchymal stem cell-derived exosomes ameliorate knee osteoarthritis by promoting cartilage regeneration and alleviating pain in rats Article Snippet: .. The cells were then washed three times with PBS, with each wash lasting 5 min. After blocking with bovine serum albumin (BSA) for 20 min at room temperature and subsequent washing with PBS, the cells were incubated overnight at 4 °C with primary antibodies against iNOS (Servicebio, China), COX2 (Servicebio, China), Collagen II (Bisso, China), aggrecan (Bisso, China), ADAMTS-5 (Bisso, China), Article Title: Partial meniscus allograft transplantation preserving the recipient’s anterior root: a comparative study in a rabbit model Article Snippet: To block endogenous peroxidase activity, sections were incubated in 3% methanol hydrogen peroxide solution for 20 min in the dark, followed by blocking with goat serum (G1208, Servicebio, Wuhan) for 30 min at room temperature. .. After blocking, sections were incubated overnight at 4 °C with the following primary antibodies: COL-1 (1:500, GB154197, Servicebio, Wuhan), COL-2 (1:1000, GB12021, Servicebio, Wuhan), MMP-9 (1:200, 10375-2-AP, Proteintech, Wuhan), and Article Title: Partial meniscus allograft transplantation preserving the recipient's anterior root: a comparative study in a rabbit model. Article Snippet: To block endogenous peroxidase activity, sections were incubated in 3% methanol hydrogen peroxide solution for 20 min in the dark, followed by blocking with goat serum (G1208, Servicebio, Wuhan) for 30 min at room temperature. .. After blocking, sections were incubated overnight at 4°C with the following primary antibodies: COL-1 (1:500, GB154197, Servicebio, Wuhan), COL-2 (1:1000, GB12021, Servicebio, Wuhan), MMP-9 (1:200, 10375-2-AP, Proteintech, Wuhan), and Article Title: Denosumab attenuates knee osteoarthritis progression by inhibiting synovial inflammation via the RANK/TRAF6/FSTL1 signalling Article Snippet: .. The membrane was then incubated overnight at 4 °C with the following primary antibodies against the following proteins: RANK (1:1,000, Abclonal, A13382), RANKL (1:1,000, Proteintech, 23408-1-AP), Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression Article Snippet: For immunohistochemistry (IHC), tissue sections underwent sequential antigen retrieval and blocking using a high-power microwave treatment, endogenous peroxidase blocker (Biocare Medical, USA), and 5 % sheep serum (Beyotime, China). .. Sections were then incubated with primary antibodies against Collagen II (28459-1-AP; 1:200; Proteintech, USA), Aggrecan (13880-1-AP; 1:100; Proteintech), MMP-3 (17873-1-AP; 1:200; Proteintech), and other:Article Title: Protopine attenuates hepatic fibrosis via HDAC6-mediated NLRP3 inflammasome activation. Article Snippet: Hepatic fibrosis is a central pathological feature of chronic liver diseases, but effective pharmacological interventions remain limited.. Protopine (PTP), a naturally isoquinoline alkaloid compound, has been reported to exhibit anti-inflammatory and hepatoprotective properties.. However, its specific role and mechanisms in hepatic fibrosis remain unclear. Blocking Assay:Article Title: Hypoxia-preconditioned bone marrow mesenchymal stem cell-derived exosomes ameliorate knee osteoarthritis by promoting cartilage regeneration and alleviating pain in rats Article Snippet: .. The cells were then washed three times with PBS, with each wash lasting 5 min. After blocking with bovine serum albumin (BSA) for 20 min at room temperature and subsequent washing with PBS, the cells were incubated overnight at 4 °C with primary antibodies against iNOS (Servicebio, China), COX2 (Servicebio, China), Collagen II (Bisso, China), aggrecan (Bisso, China), ADAMTS-5 (Bisso, China), Article Title: Partial meniscus allograft transplantation preserving the recipient’s anterior root: a comparative study in a rabbit model Article Snippet: To block endogenous peroxidase activity, sections were incubated in 3% methanol hydrogen peroxide solution for 20 min in the dark, followed by blocking with goat serum (G1208, Servicebio, Wuhan) for 30 min at room temperature. .. After blocking, sections were incubated overnight at 4 °C with the following primary antibodies: COL-1 (1:500, GB154197, Servicebio, Wuhan), COL-2 (1:1000, GB12021, Servicebio, Wuhan), MMP-9 (1:200, 10375-2-AP, Proteintech, Wuhan), and Article Title: Partial meniscus allograft transplantation preserving the recipient's anterior root: a comparative study in a rabbit model. Article Snippet: To block endogenous peroxidase activity, sections were incubated in 3% methanol hydrogen peroxide solution for 20 min in the dark, followed by blocking with goat serum (G1208, Servicebio, Wuhan) for 30 min at room temperature. .. After blocking, sections were incubated overnight at 4°C with the following primary antibodies: COL-1 (1:500, GB154197, Servicebio, Wuhan), COL-2 (1:1000, GB12021, Servicebio, Wuhan), MMP-9 (1:200, 10375-2-AP, Proteintech, Wuhan), and Membrane:Article Title: Denosumab attenuates knee osteoarthritis progression by inhibiting synovial inflammation via the RANK/TRAF6/FSTL1 signalling Article Snippet: .. The membrane was then incubated overnight at 4 °C with the following primary antibodies against the following proteins: RANK (1:1,000, Abclonal, A13382), RANKL (1:1,000, Proteintech, 23408-1-AP), |
