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Proteintech mmp 13
Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 <t>and</t> <t>MMP-13</t> in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.
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1) Product Images from "Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression"

Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression

Journal: Non-coding RNA Research

doi: 10.1016/j.ncrna.2025.11.007

Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.
Figure Legend Snippet: Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.

Techniques Used: In Vivo, Staining, TUNEL Assay, Immunohistochemistry, Expressing

Related Articles

Incubation:

Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression
Article Snippet: For immunohistochemistry (IHC), tissue sections underwent sequential antigen retrieval and blocking using a high-power microwave treatment, endogenous peroxidase blocker (Biocare Medical, USA), and 5 % sheep serum (Beyotime, China). .. Sections were then incubated with primary antibodies against Collagen II (28459-1-AP; 1:200; Proteintech, USA), Aggrecan (13880-1-AP; 1:100; Proteintech), MMP-3 (17873-1-AP; 1:200; Proteintech), and MMP-13 (18165-1-AP; 1:100; Proteintech), followed by the corresponding secondary antibodies. .. HE, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), and IHC stained sections were scanned using a Pannoramic MIDI (3D HISTECH, Hungary), whereas EdU-stained sections were acquired using a BX53 microscope.

Article Title: Hypoxia-preconditioned bone marrow mesenchymal stem cell-derived exosomes ameliorate knee osteoarthritis by promoting cartilage regeneration and alleviating pain in rats
Article Snippet: .. The sections were blocked with 10 % fetal bovine serum (FBS) for 1 h at room temperature, then incubated overnight at 4 °C with primary antibodies against iNOS (Servicebio, China), COX2 (ProteinTech Group, China), Collagen II (Bioss, China), aggrecan (Bioss, China), MMP-13 (ProteinTech Group, China), and ADAMTS-5 (Zenbio, China). ..

Article Title: Hypoxia-preconditioned bone marrow mesenchymal stem cell-derived exosomes ameliorate knee osteoarthritis by promoting cartilage regeneration and alleviating pain in rats
Article Snippet: .. The cells were then washed three times with PBS, with each wash lasting 5 min. After blocking with bovine serum albumin (BSA) for 20 min at room temperature and subsequent washing with PBS, the cells were incubated overnight at 4 °C with primary antibodies against iNOS (Servicebio, China), COX2 (Servicebio, China), Collagen II (Bisso, China), aggrecan (Bisso, China), ADAMTS-5 (Bisso, China), MMP-13 (Affinity, USA), p16 (Abcam, USA), p21 (Affinity, USA), and p53 (Proteintech, USA). ..

Article Title: Partial meniscus allograft transplantation preserving the recipient’s anterior root: a comparative study in a rabbit model
Article Snippet: To block endogenous peroxidase activity, sections were incubated in 3% methanol hydrogen peroxide solution for 20 min in the dark, followed by blocking with goat serum (G1208, Servicebio, Wuhan) for 30 min at room temperature. .. After blocking, sections were incubated overnight at 4 °C with the following primary antibodies: COL-1 (1:500, GB154197, Servicebio, Wuhan), COL-2 (1:1000, GB12021, Servicebio, Wuhan), MMP-9 (1:200, 10375-2-AP, Proteintech, Wuhan), and MMP-13 (1:200, 18165-1-AP, Proteintech, Wuhan). ..

Article Title: Partial meniscus allograft transplantation preserving the recipient's anterior root: a comparative study in a rabbit model.
Article Snippet: To block endogenous peroxidase activity, sections were incubated in 3% methanol hydrogen peroxide solution for 20 min in the dark, followed by blocking with goat serum (G1208, Servicebio, Wuhan) for 30 min at room temperature. .. After blocking, sections were incubated overnight at 4°C with the following primary antibodies: COL-1 (1:500, GB154197, Servicebio, Wuhan), COL-2 (1:1000, GB12021, Servicebio, Wuhan), MMP-9 (1:200, 10375-2-AP, Proteintech, Wuhan), and MMP-13 (1:200, 18165-1-AP, Proteintech, Wuhan). ..

Article Title: Denosumab attenuates knee osteoarthritis progression by inhibiting synovial inflammation via the RANK/TRAF6/FSTL1 signalling
Article Snippet: .. The membrane was then incubated overnight at 4 °C with the following primary antibodies against the following proteins: RANK (1:1,000, Abclonal, A13382), RANKL (1:1,000, Proteintech, 23408-1-AP), MMP-13 (1:1,000, Proteintech, 18165-1-AP), ADAMTS-5 (1:1,000, Boster, A02802-1), Col2a1 (1:1,000, Bioss, bs-0709R), Col10a1 (1:1,000, Abcam, ab182563), TNF-α (1:1,000, Abclonal, A11534), IL-1β (1:1,000, Affinity Biosciences, AF5103), CD86 (1:1,000, Proteintech, 13395-1-AP), Arg1 (1:1,000, Affinity Biosciences, DF6657), FSTL1 (1:1,000, Proteintech, 20182-1-AP), TRAF6 (1:1,000, Santa Cruz, sc8409), IκB-α (1:2,000, Proteintech, 10268-1-AP), p-IκB-α (1:1000, CST, 2859 T), p-65 (1:1,000, Proteintech, 10745-1-AP), p-50 (1:1,000, Proteintech, 14220-1-AP) and H3 (1:1,000, ab32356, Abcam); GAPDH (1:3,000, Proteintech, 60004-1-Ig) was used as the internal reference. ..

Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression
Article Snippet: For immunohistochemistry (IHC), tissue sections underwent sequential antigen retrieval and blocking using a high-power microwave treatment, endogenous peroxidase blocker (Biocare Medical, USA), and 5 % sheep serum (Beyotime, China). .. Sections were then incubated with primary antibodies against Collagen II (28459-1-AP; 1:200; Proteintech, USA), Aggrecan (13880-1-AP; 1:100; Proteintech), MMP-3 (17873-1-AP; 1:200; Proteintech), and MMP-13 (18165-1-AP; 1:100; Proteintech), followed by the corresponding secondary antibodies. .. HE, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), and IHC stained sections were scanned using a Pannoramic MIDI (3D HISTECH, Hungary), whereas EdU-stained sections were acquired using a BX53 microscope.

other:

Article Title: Protopine attenuates hepatic fibrosis via HDAC6-mediated NLRP3 inflammasome activation.
Article Snippet: Hepatic fibrosis is a central pathological feature of chronic liver diseases, but effective pharmacological interventions remain limited.. Protopine (PTP), a naturally isoquinoline alkaloid compound, has been reported to exhibit anti-inflammatory and hepatoprotective properties.. However, its specific role and mechanisms in hepatic fibrosis remain unclear.

Blocking Assay:

Article Title: Hypoxia-preconditioned bone marrow mesenchymal stem cell-derived exosomes ameliorate knee osteoarthritis by promoting cartilage regeneration and alleviating pain in rats
Article Snippet: .. The cells were then washed three times with PBS, with each wash lasting 5 min. After blocking with bovine serum albumin (BSA) for 20 min at room temperature and subsequent washing with PBS, the cells were incubated overnight at 4 °C with primary antibodies against iNOS (Servicebio, China), COX2 (Servicebio, China), Collagen II (Bisso, China), aggrecan (Bisso, China), ADAMTS-5 (Bisso, China), MMP-13 (Affinity, USA), p16 (Abcam, USA), p21 (Affinity, USA), and p53 (Proteintech, USA). ..

Article Title: Partial meniscus allograft transplantation preserving the recipient’s anterior root: a comparative study in a rabbit model
Article Snippet: To block endogenous peroxidase activity, sections were incubated in 3% methanol hydrogen peroxide solution for 20 min in the dark, followed by blocking with goat serum (G1208, Servicebio, Wuhan) for 30 min at room temperature. .. After blocking, sections were incubated overnight at 4 °C with the following primary antibodies: COL-1 (1:500, GB154197, Servicebio, Wuhan), COL-2 (1:1000, GB12021, Servicebio, Wuhan), MMP-9 (1:200, 10375-2-AP, Proteintech, Wuhan), and MMP-13 (1:200, 18165-1-AP, Proteintech, Wuhan). ..

Article Title: Partial meniscus allograft transplantation preserving the recipient's anterior root: a comparative study in a rabbit model.
Article Snippet: To block endogenous peroxidase activity, sections were incubated in 3% methanol hydrogen peroxide solution for 20 min in the dark, followed by blocking with goat serum (G1208, Servicebio, Wuhan) for 30 min at room temperature. .. After blocking, sections were incubated overnight at 4°C with the following primary antibodies: COL-1 (1:500, GB154197, Servicebio, Wuhan), COL-2 (1:1000, GB12021, Servicebio, Wuhan), MMP-9 (1:200, 10375-2-AP, Proteintech, Wuhan), and MMP-13 (1:200, 18165-1-AP, Proteintech, Wuhan). ..

Membrane:

Article Title: Denosumab attenuates knee osteoarthritis progression by inhibiting synovial inflammation via the RANK/TRAF6/FSTL1 signalling
Article Snippet: .. The membrane was then incubated overnight at 4 °C with the following primary antibodies against the following proteins: RANK (1:1,000, Abclonal, A13382), RANKL (1:1,000, Proteintech, 23408-1-AP), MMP-13 (1:1,000, Proteintech, 18165-1-AP), ADAMTS-5 (1:1,000, Boster, A02802-1), Col2a1 (1:1,000, Bioss, bs-0709R), Col10a1 (1:1,000, Abcam, ab182563), TNF-α (1:1,000, Abclonal, A11534), IL-1β (1:1,000, Affinity Biosciences, AF5103), CD86 (1:1,000, Proteintech, 13395-1-AP), Arg1 (1:1,000, Affinity Biosciences, DF6657), FSTL1 (1:1,000, Proteintech, 20182-1-AP), TRAF6 (1:1,000, Santa Cruz, sc8409), IκB-α (1:2,000, Proteintech, 10268-1-AP), p-IκB-α (1:1000, CST, 2859 T), p-65 (1:1,000, Proteintech, 10745-1-AP), p-50 (1:1,000, Proteintech, 14220-1-AP) and H3 (1:1,000, ab32356, Abcam); GAPDH (1:3,000, Proteintech, 60004-1-Ig) was used as the internal reference. ..



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Efficacy of mechanically sensitized OBNC microspheres in the treatment of osteoarthritis. A) Diagram of OBNC microspheres self-adaptive filling of a cartilage injury site to promote cartilage repair and regeneration. B) Representative images of H&E staining. C) OARSI scores of articular cartilage in each group. D) Representative images of safranin O-fast green staining. E) Relative glycosaminoglycan (GAG) content in each group. F) Representative sections showing TUNEL staining for apoptotic cells. G) Quantification of TUNEL-positive cells. H) Representative sections showing Collagen II staining for chondrocytes. I) Relative collagen II expression in each group. J) Representative sections showing MMP-13 staining of chondrocytes. K) Relative MMP-13 expression in each group. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01).

Journal: Bioactive Materials

Article Title: Mechanically sensitized hydrogel microspheres trigger membrane receptor switch for cartilage repair

doi: 10.1016/j.bioactmat.2026.03.017

Figure Lengend Snippet: Efficacy of mechanically sensitized OBNC microspheres in the treatment of osteoarthritis. A) Diagram of OBNC microspheres self-adaptive filling of a cartilage injury site to promote cartilage repair and regeneration. B) Representative images of H&E staining. C) OARSI scores of articular cartilage in each group. D) Representative images of safranin O-fast green staining. E) Relative glycosaminoglycan (GAG) content in each group. F) Representative sections showing TUNEL staining for apoptotic cells. G) Quantification of TUNEL-positive cells. H) Representative sections showing Collagen II staining for chondrocytes. I) Relative collagen II expression in each group. J) Representative sections showing MMP-13 staining of chondrocytes. K) Relative MMP-13 expression in each group. (ns: non-significant, ∗ P < 0.05, ∗∗ P < 0.01).

Article Snippet: Antigen retrieval was performed by microwaving in citrate buffer for 10 min. Endogenous peroxidase activity was blocked by incubating the sections with 3% hydrogen peroxide for 10 min, and non-specific binding was blocked with 3% BSA for 1 h. The sections were incubated overnight at 4 °C with primary antibodies to Collagen II and MMP-13 (ServiceBio, China), washed, and incubated for 1 h with HRP-labeled secondary antibodies (ServiceBio, China).

Techniques: Staining, TUNEL Assay, Expressing

Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.

Journal: Non-coding RNA Research

Article Title: Hsa_circ_0101645 contributes to excessive autophagy and apoptosis in intervertebral disc degeneration by acting as a miR-1304-5p sponge modulating BNIP3 expression

doi: 10.1016/j.ncrna.2025.11.007

Figure Lengend Snippet: Hsa_circ_0101645 accelerating the IVDD process in vivo . A: Diagram of the animal procedure for this study. B: Grouping information for this section. C: Representative X-rays of each group of rats. Statistical graph demonstrating the disc height index (DHI) changes for L4/5 in each group of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). D: HE staining exhibiting pathological changes of CEP, NP, and AP in IVD in each group of rats (N = 6). Scale bar: 500 μm. E: EdU staining was used to detect cell proliferation in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. F: TUNEL (white light) staining exhibiting TUNEL-positive cells in IVD tissues of rats (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 50 μm. G: The effect of hsa_circ_0101645 on the protein levels of Collagen Ⅱ, Aggrecan, MMP-3 and MMP-13 in IVD was observed by IHC staining (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). Scale bar: 100 μm. H-I: The expression of hsa_circ_0101645 (H) and miR-1304-5p (I) in each group of IVD tissues (N = 6) (One-way ANOVA test with Tukey's multiple comparisons test). J-K: Changes in expression of apoptosis ( J; Caspase 3, Bcl-2 and Bax) and autophagy markers ( K; LC3B, Beclin and P62) in IVD tissues (N = 3) (One-way ANOVA test with Tukey's multiple comparisons test or Kruskal-Wallis test with Dunn's multiple comparisons test). ∗ indicates P < 0.05.

Article Snippet: Sections were then incubated with primary antibodies against Collagen II (28459-1-AP; 1:200; Proteintech, USA), Aggrecan (13880-1-AP; 1:100; Proteintech), MMP-3 (17873-1-AP; 1:200; Proteintech), and MMP-13 (18165-1-AP; 1:100; Proteintech), followed by the corresponding secondary antibodies.

Techniques: In Vivo, Staining, TUNEL Assay, Immunohistochemistry, Expressing